Structure Evaluation of IroN for Designing a Vaccine against Escherichia Coli, an In Silico Approach
نویسندگان
چکیده مقاله:
Introduction: Some strains of Escherichia Coli, including intestinal pathogenic strains, commensal strains, and extra intestinal pathogenic E. coli (ExPEC) have a significant impact on human health status. A standard vaccine designed based on conserved epitopes can stimulate a protective immune response against these pathogens. Additionally, enhanced expression at the infection site as a pathogenesis factor in disease is crucial for an ideal vaccine candidate. The IroN protein plays a role in severe infections of E. coli. Hence, this protein will assist in developing the novel and more efficient treatments for E. coli related infections. A better understanding of protein tertiary structure can help to percept their functions and also their interactions with other molecules. There is a growing interest in using bioinformatics tool to make accurate predictions about the functional, immunological, and biochemical features of target antigens. Method: Herein, we aimed to predict the structure of the IroN protein upon its folding and determine their immunological properties. Results: In the present study, using bioinformatics analyses, we identified the highly antigenic regions of IroN protein. Our designed vaccine candidate had the highest immunological properties and folded into a typical beta-barrel structure. Conclusion: The approach of assigning structural and immunological properties of the target antigen to design the vaccine candidate could be deployed as an efficient strategy to circumvent the challenges ahead of empirical methods without dealing with ethical concerns of animal usage and human participants. Although the obtained results are promising, further experimental studies could bring about more insights on the efficiency of the designed vaccine.
منابع مشابه
A Chimeric Vaccine Consisting of Highly Immunogenic Regions form Escherichia coli Iron Regulated Outer-Membrane Proteins: An In Silico Approach
متن کامل
Designing a polytopic complex vaccine candidate against Gallibacterium anatis: an In-silico study
The haemolytic biovar of Gallibacterium anatis (G. anatis) is responsible for urogenital, gastrointestinal, and respiratory diseases in chickens. There are numerous reports on the resistance of G. anatis to antibiotics and recurrence of the disease, which raise concerns about antimicrobial treatment efficiency. Vaccination has been considered as the most feasibl...
متن کاملA DNA Vaccine against Escherichia coli O157:H7
Background: Infection with Escherichia coli O157:H7 rarely leads to bloody diarrhea and causes hemolytic uremic syndrome with renal failure that can be deadly dangerous. Intimin, translocated Intimin receptor (Tir), and enterohemorrhagic E. coli (EHEC) secreted protein A (EspA) proteins are the virulence factors expressed by locus of enterocyte effacement locus of EHEC. This bacterium needs Es...
متن کاملImmunogenic Evaluation of Bivalent Vaccine Candidate against Enterohemorrhagic and Enterotoxigenic Escherichia coli
Background: Caused by bacterial, viral, and parasitic pathogens, diarrhea is the second leading cause of death among children under five. Two strains of E. coli, namely Enterotoxigenic, ETEC and Enterohemorrhagic EHEC are the most important causes of this disease in developing countries. EHEC is a major causative agent of bloody diarrhea and hemorrhag...
متن کاملdesigning a novel multi-epitope dna- based vaccine against tuberculosis: in silico approach
conclusions the results revealed that the novel multi-epitope dna construct could be an effective candidate in tuberculosis vaccine development, and it is qualified to investigate its potential to induce cd4 and cd8 t- cell immune response in the experimental animal model. results mhc class i-and class ii-predicted epitopes showed a high potential to binding to human hlas alleles, with global b...
متن کاملDesigning a Construct of Chimeric Multi-Epitopes Protein for Contraceptive Vaccine in Mice: An Immunoinformatics and In Silico Study
Background: Contraceptive vaccines (CVs) can be used as a valuable and alternative method for the prevention of gestation in humans and animals. These vaccines can have several targets, such as superficial sperm proteins. Vaccines based on sperm antigens are quite efficacious to create a contraceptive effect. However, multi-epitope vaccines are more effective in stimulating the immune system an...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 7 شماره 4
صفحات 93- 106
تاریخ انتشار 2019-10
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023